Cytokines

The application of cytokine drugs such as interferon is one of the most active areas in biomedical research. At present, cytokine preparations such as human genetically engineered interferon and interleukin have been widely used in the world to treat more than 30 acute and chronic viral diseases (including new coronavirus infection) and tumors; and in the research and development of the latest vaccines such as AIDS vaccine and tumor vaccine, cytokines have been It is used as an immune adjuvant or an immune enhancer. However, the most significant defect in the clinical application of cytokine drugs such as genetically engineered interferon is that the half-life of drug metabolism is too short and can only be used by injection. At present, the human genetically engineered interferon developed by world-renowned pharmaceutical companies such as Roche and Amgen has developed into the second-generation long-acting interferon, which solves the problem of short drug metabolism half-life. Compared with the first-generation genetically engineered interferon, it has been significantly improved, but the research on animal interferon is relatively lagging behind, and it is still at the level of the first-generation genetically engineered interferon.

The team used genetic engineering technology to solve the limitation of the short half-life of traditional cytokines such as recombinant interferon, and extended the half-life from 4h to 12h. While the efficacy is greatly enhanced, the protein stability is increased, so that the traditional recombinant interferon and other cytokines can only be used by injection and can be extended to oral, spray and other uses.

Table 1 Advantages of the second-generation long-acting interferon cytokine immune enhancer

1. Second-generation long-acting interferon (pig, poultry, cattle, sheep, dogs, cats)

Interferon (IFN) is a cytokine, a class of glycoproteins induced by viruses or other interferon-inducing agents to induce the production of human or animal cells. regulation and many other biological activities. At present, human genetically engineered interferon preparations have been widely used in the world to treat more than 30 acute and chronic viral diseases (including new coronavirus infection) and tumors, and it is the second largest biological protein drug in the world. 

Figure 1 Mechanism of action of second-generation long-acting interferon

2. Novel targeted anti-inflammatory factor

Anti-inflammatory drugs are the second largest class of drugs in clinical practice after anti-infective drugs. Currently, common clinical anti-inflammatory drugs include non-steroidal anti-inflammatory drugs, hormones and some traditional Chinese medicines. This project solves the problems of application cost and yield of animal tumor necrosis factor alpha targeted inhibitors in the veterinary field through the unique recombinant protein high-efficiency supernatant soluble expression technology.